6EQO
Tri-functional propionyl-CoA synthase of Erythrobacter sp. NAP1 with bound NADP+ and phosphomethylphosphonic acid adenylate ester
Summary for 6EQO
| Entry DOI | 10.2210/pdb6eqo/pdb |
| Descriptor | Acetyl-coenzyme A synthetase, PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (4 entities in total) |
| Functional Keywords | 3-hydroxpropionyl-coa synthetase, 3-hydroxpropionyl-coa dehydratase, acrylyl-coa reductase, central carbon metabolism, carbon dioxode fixation, 3-hydroxypropionate bi-cycle, natural fusion enzyme, substrate channeling, oxidoreductase |
| Biological source | Erythrobacter sp. NAP1 |
| Total number of polymer chains | 2 |
| Total formula weight | 403752.44 |
| Authors | Zarzycki, J.,Bernhardsgruetter, I.,Voegeli, B.,Wagner, T.,Engilberge, S.,Girard, E.,Shima, S.,Erb, T.J. (deposition date: 2017-10-13, release date: 2018-10-24, Last modification date: 2024-05-08) |
| Primary citation | Bernhardsgrutter, I.,Vogeli, B.,Wagner, T.,Peter, D.M.,Cortina, N.S.,Kahnt, J.,Bange, G.,Engilberge, S.,Girard, E.,Riobe, F.,Maury, O.,Shima, S.,Zarzycki, J.,Erb, T.J. The multicatalytic compartment of propionyl-CoA synthase sequesters a toxic metabolite. Nat. Chem. Biol., 14:1127-1132, 2018 Cited by PubMed Abstract: Cells must cope with toxic or reactive intermediates formed during metabolism. One coping strategy is to sequester reactions that produce such intermediates within specialized compartments or tunnels connecting different active sites. Here, we show that propionyl-CoA synthase (PCS), an ∼ 400-kDa homodimer, three-domain fusion protein and the key enzyme of the 3-hydroxypropionate bi-cycle for CO fixation, sequesters its reactive intermediate acrylyl-CoA. Structural analysis showed that PCS forms a multicatalytic reaction chamber. Kinetic analysis suggested that access to the reaction chamber and catalysis are synchronized by interdomain communication. The reaction chamber of PCS features three active sites and has a volume of only 33 nm. As one of the smallest multireaction chambers described in biology, PCS may inspire the engineering of a new class of dynamically regulated nanoreactors. PubMed: 30374166DOI: 10.1038/s41589-018-0153-x PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
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