6EM1

State C (Nsa1-TAP Flag-Ytm1) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes

これはPDB形式変換不可エントリーです。

6EM1 の概要

• エントリーDOI: 10.2210/pdb6em1/pdb
• EMDBエントリー: 3893
• 分子名称: Ribosome production factor 1, Nuclear GTP-binding protein NUG1, Ribosome biogenesis protein RLP24, ... (44 entities in total)
• 機能のキーワード: large subunit biogenesis nucleolus, ribosome
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• 細胞内の位置: Nucleus, nucleolus : P38805 P40007 P33201 P38779 Q04660 P35178 Q03532 A6ZV85 P40693 P53136 Q08235 Q02892 P36049 P40078; Nucleus : P40010 P10962; Cytoplasm : Q07915 Q12522 P14126 P10664 P38061 Q02326 P05737 P05744 P17076 P0CX84 P05738 O14455 P49166 Q12690 P36105 P05748 P26784 P05740 P0CX49 P0CX23 P0CX41 P05743 P53927
• タンパク質・核酸の鎖数: 43
• 化学式量合計: 2482067.58

構造登録者

Kater, L.,Cheng, J.,Barrio-Garcia, C.,Hurt, E.,Beckmann, R. (登録日: 2017-10-01, 公開日: 2017-12-27, 最終更新日: 2025-10-01)

主引用文献

Kater, L.,Thoms, M.,Barrio-Garcia, C.,Cheng, J.,Ismail, S.,Ahmed, Y.L.,Bange, G.,Kressler, D.,Berninghausen, O.,Sinning, I.,Hurt, E.,Beckmann, R.
Visualizing the Assembly Pathway of Nucleolar Pre-60S Ribosomes.
Cell, 171:1599-1610.e14, 2017

PubMed Abstract: Eukaryotic 60S ribosomal subunits are comprised of three rRNAs and ∼50 ribosomal proteins. The initial steps of their formation take place in the nucleolus, but, owing to a lack of structural information, this process is poorly understood. Using cryo-EM, we solved structures of early 60S biogenesis intermediates at 3.3 Å to 4.5 Å resolution, thereby providing insights into their sequential folding and assembly pathway. Besides revealing distinct immature rRNA conformations, we map 25 assembly factors in six different assembly states. Notably, the Nsa1-Rrp1-Rpf1-Mak16 module stabilizes the solvent side of the 60S subunit, and the Erb1-Ytm1-Nop7 complex organizes and connects through Erb1's meandering N-terminal extension, eight assembly factors, three ribosomal proteins, and three 25S rRNA domains. Our structural snapshots reveal the order of integration and compaction of the six major 60S domains within early nucleolar 60S particles developing stepwise from the solvent side around the exit tunnel to the central protuberance.

PubMed: 29245012
DOI: 10.1016/j.cell.2017.11.039

実験手法

ELECTRON MICROSCOPY (3.6 Å)