6EGC

Single-chain version of 2L4HC2_23 (PDB 5J0K)

6EGC の概要

• エントリーDOI: 10.2210/pdb6egc/pdb
• 分子名称: SC_2L4HC2_23 (2 entities in total)
• 機能のキーワード: computational design, helical bundle, de novo protein, coiled-coil
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19030.60

構造登録者

Bick, M.J.,Chen, Z.,DiMaio, F. (登録日: 2018-08-19, 公開日: 2019-05-29, 最終更新日: 2024-04-03)

主引用文献

Chen, Z.,Johnson, M.C.,Chen, J.,Bick, M.J.,Boyken, S.E.,Lin, B.,De Yoreo, J.J.,Kollman, J.M.,Baker, D.,DiMaio, F.
Self-Assembling 2D Arrays with de Novo Protein Building Blocks.
J.Am.Chem.Soc., 141:8891-8895, 2019

PubMed Abstract: Modular self-assembly of biomolecules in two dimensions (2D) is straightforward with DNA but has been difficult to realize with proteins, due to the lack of modular specificity similar to Watson-Crick base pairing. Here we describe a general approach to design 2D arrays using de novo designed pseudosymmetric protein building blocks. A homodimeric helical bundle was reconnected into a monomeric building block, and the surface was redesigned in Rosetta to enable self-assembly into a 2D array in the C12 layer symmetry group. Two out of ten designed arrays assembled to micrometer scale under negative stain electron microscopy, and displayed the designed lattice geometry with assembly size up to 100 nm under atomic force microscopy. The design of 2D arrays with pseudosymmetric building blocks is an important step toward the design of programmable protein self-assembly via pseudosymmetric patterning of orthogonal binding interfaces.

PubMed: 31050411
DOI: 10.1021/jacs.9b01978

実験手法

X-RAY DIFFRACTION (1.74 Å)