6EF8

Cryo-EM of the OmcS nanowires from Geobacter sulfurreducens

6EF8 の概要

• エントリーDOI: 10.2210/pdb6ef8/pdb
• EMDBエントリー: 9046
• 分子名称: C-type cytochrome OmcS, HEME C (2 entities in total)
• 機能のキーワード: helical symmetry, cytochrome nanowire, cytochrome c3, filament, electron transport
• 由来する生物種: Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 326879.18

構造登録者

Wang, F.,Gu, Y.,Egelman, E.H.,Malvankar, N.S. (登録日: 2018-08-16, 公開日: 2019-04-10, 最終更新日: 2024-10-16)

主引用文献

Wang, F.,Gu, Y.,O'Brien, J.P.,Yi, S.M.,Yalcin, S.E.,Srikanth, V.,Shen, C.,Vu, D.,Ing, N.L.,Hochbaum, A.I.,Egelman, E.H.,Malvankar, N.S.
Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers.
Cell, 177:361-369.e10, 2019

PubMed Abstract: Long-range (>10 μm) transport of electrons along networks of Geobacter sulfurreducens protein filaments, known as microbial nanowires, has been invoked to explain a wide range of globally important redox phenomena. These nanowires were previously thought to be type IV pili composed of PilA protein. Here, we report a 3.7 Å resolution cryoelectron microscopy structure, which surprisingly reveals that, rather than PilA, G. sulfurreducens nanowires are assembled by micrometer-long polymerization of the hexaheme cytochrome OmcS, with hemes packed within ∼3.5-6 Å of each other. The inter-subunit interfaces show unique structural elements such as inter-subunit parallel-stacked hemes and axial coordination of heme by histidines from neighboring subunits. Wild-type OmcS filaments show 100-fold greater conductivity than other filaments from a ΔomcS strain, highlighting the importance of OmcS to conductivity in these nanowires. This structure explains the remarkable capacity of soil bacteria to transport electrons to remote electron acceptors for respiration and energy sharing.

PubMed: 30951668
DOI: 10.1016/j.cell.2019.03.029

実験手法

ELECTRON MICROSCOPY (3.7 Å)