6E9X

DHF91 filament

6E9X の概要

• エントリーDOI: 10.2210/pdb6e9x/pdb
• EMDBエントリー: 9016 9017 9018 9019 9020 9021
• 分子名称: DHF91 filament (1 entity in total)
• 機能のキーワード: protein design, filament, protein fibril
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 21
• 化学式量合計: 374442.75

構造登録者

Lynch, E.M.,Shen, H.,Fallas, J.A.,Kollman, J.M.,Baker, D. (登録日: 2018-08-01, 公開日: 2018-11-21, 最終更新日: 2024-03-13)

主引用文献

Shen, H.,Fallas, J.A.,Lynch, E.,Sheffler, W.,Parry, B.,Jannetty, N.,Decarreau, J.,Wagenbach, M.,Vicente, J.J.,Chen, J.,Wang, L.,Dowling, Q.,Oberdorfer, G.,Stewart, L.,Wordeman, L.,De Yoreo, J.,Jacobs-Wagner, C.,Kollman, J.,Baker, D.
De novo design of self-assembling helical protein filaments.
Science, 362:705-709, 2018

PubMed Abstract: We describe a general computational approach to designing self-assembling helical filaments from monomeric proteins and use this approach to design proteins that assemble into micrometer-scale filaments with a wide range of geometries in vivo and in vitro. Cryo-electron microscopy structures of six designs are close to the computational design models. The filament building blocks are idealized repeat proteins, and thus the diameter of the filaments can be systematically tuned by varying the number of repeat units. The assembly and disassembly of the filaments can be controlled by engineered anchor and capping units built from monomers lacking one of the interaction surfaces. The ability to generate dynamic, highly ordered structures that span micrometers from protein monomers opens up possibilities for the fabrication of new multiscale metamaterials.

PubMed: 30409885
DOI: 10.1126/science.aau3775

実験手法

ELECTRON MICROSCOPY (7.8 Å)