6E9V

DHF79 filament

Summary for 6E9V

• Entry DOI: 10.2210/pdb6e9v/pdb
• EMDB information: 9016 9017 9018 9019 9020 9021
• Descriptor: DHF79 filament (1 entity in total)
• Functional Keywords: protein design, filament, protein fibril
• Biological source: synthetic construct
• Total number of polymer chains: 26
• Total formula weight: 600910.13

Authors

Lynch, E.M.,Shen, H.,Fallas, J.A.,Kollman, J.M.,Baker, D. (deposition date: 2018-08-01, release date: 2018-11-21, Last modification date: 2024-03-13)

Primary citation

Shen, H.,Fallas, J.A.,Lynch, E.,Sheffler, W.,Parry, B.,Jannetty, N.,Decarreau, J.,Wagenbach, M.,Vicente, J.J.,Chen, J.,Wang, L.,Dowling, Q.,Oberdorfer, G.,Stewart, L.,Wordeman, L.,De Yoreo, J.,Jacobs-Wagner, C.,Kollman, J.,Baker, D.
De novo design of self-assembling helical protein filaments.
Science, 362:705-709, 2018

PubMed Abstract: We describe a general computational approach to designing self-assembling helical filaments from monomeric proteins and use this approach to design proteins that assemble into micrometer-scale filaments with a wide range of geometries in vivo and in vitro. Cryo-electron microscopy structures of six designs are close to the computational design models. The filament building blocks are idealized repeat proteins, and thus the diameter of the filaments can be systematically tuned by varying the number of repeat units. The assembly and disassembly of the filaments can be controlled by engineered anchor and capping units built from monomers lacking one of the interaction surfaces. The ability to generate dynamic, highly ordered structures that span micrometers from protein monomers opens up possibilities for the fabrication of new multiscale metamaterials.

PubMed: 30409885
DOI: 10.1126/science.aau3775

Experimental method

ELECTRON MICROSCOPY (6.9 Å)