6E9F

EsCas13d-crRNA-target RNA ternary complex

6E9F の概要

• エントリーDOI: 10.2210/pdb6e9f/pdb
• EMDBエントリー: 9013 9014 9015
• 分子名称: EsCas13d, crRNA (52-MER), RNA (27-MER), ... (4 entities in total)
• 機能のキーワード: crispr-cas, rnase, complex, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: [Eubacterium] siraeum DSM 15702; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 135835.99

構造登録者

Zhang, C.,Lyumkis, D. (登録日: 2018-08-01, 公開日: 2018-10-03, 最終更新日: 2024-03-13)

主引用文献

Zhang, C.,Konermann, S.,Brideau, N.J.,Lotfy, P.,Wu, X.,Novick, S.J.,Strutzenberg, T.,Griffin, P.R.,Hsu, P.D.,Lyumkis, D.
Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d.
Cell, 175:212-223.e17, 2018

PubMed Abstract: CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryotic adaptive immunity and have been tailored for broad genetic engineering applications. Type VI-D CRISPR systems contain the smallest known family of single effector Cas enzymes, and their signature Cas13d ribonuclease employs guide RNAs to cleave matching target RNAs. To understand the molecular basis for Cas13d function and explain its compact molecular architecture, we resolved cryoelectron microscopy structures of Cas13d-guide RNA binary complex and Cas13d-guide-target RNA ternary complex to 3.4 and 3.3 Å resolution, respectively. Furthermore, a 6.5 Å reconstruction of apo Cas13d combined with hydrogen-deuterium exchange revealed conformational dynamics that have implications for RNA scanning. These structures, together with biochemical and cellular characterization, provide insights into its RNA-guided, RNA-targeting mechanism and delineate a blueprint for the rational design of improved transcriptome engineering technologies.

PubMed: 30241607
DOI: 10.1016/j.cell.2018.09.001

実験手法

ELECTRON MICROSCOPY (3.3 Å)