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6E84

Crystal structure of the Corn aptamer in complex with TO

Summary for 6E84
Entry DOI10.2210/pdb6e84/pdb
Related6E81
DescriptorRNA (36-MER), POTASSIUM ION, 1-methyl-4-[(Z)-(3-methyl-1,3-benzothiazol-2(3H)-ylidene)methyl]quinolin-1-ium (3 entities in total)
Functional Keywordspolyribonucleotide, fluorigenic aptamer, g-quadruplex, thiazole orange, rna
Biological sourcesynthetic construct
Total number of polymer chains1
Total formula weight12138.61
Authors
Sjekloca, L.,Ferre-D'Amare, A.R. (deposition date: 2018-07-27, release date: 2019-07-31, Last modification date: 2023-10-11)
Primary citationSjekloca, L.,Ferre-D'Amare, A.R.
Binding between G Quadruplexes at the Homodimer Interface of the Corn RNA Aptamer Strongly Activates Thioflavin T Fluorescence.
Cell Chem Biol, 26:1159-, 2019
Cited by
PubMed Abstract: Thioflavin T (ThT) is widely used for the detection of amyloids. Many unrelated DNAs and RNAs that contain G-quadruplex motifs also bind ThT and strongly activate its fluorescence. To elucidate the structural basis of ThT binding to G quadruplexes and its fluorescence turn-on, we determined its co-crystal structure with the homodimeric RNA Corn, which contains two G quadruplexes. We found that two ThT molecules bind in the dimer interface, constrained by a G quartet from each protomer into a maximally fluorescent planar conformation. The unliganded Corn homodimer crystal structure reveals a collapsed fluorophore-binding site. In solution, Corn must fluctuate between this and an open, binding-competent conformation. A co-crystal structure with another benzothiazole derivate, thiazole orange (TO), also shows binding at the Corn homodimer interface. As the bound ThT and TO make no interactions with the RNA backbone, their Corn co-crystal structures likely explain their fluorescence activation upon sequence-independent DNA and RNA G-quadruplex binding.
PubMed: 31178406
DOI: 10.1016/j.chembiol.2019.04.012
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.901 Å)
Structure validation

238582

數據於2025-07-09公開中

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