6E82

Crystal structure of the Corn aptamer mutant A14U in complex with ThT

6E82 の概要

• エントリーDOI: 10.2210/pdb6e82/pdb
• 関連するPDBエントリー: 6E81
• 分子名称: RNA (36-MER), POTASSIUM ION, 2-[4-(dimethylamino)phenyl]-3,6-dimethyl-1,3-benzothiazol-3-ium (3 entities in total)
• 機能のキーワード: polyribonucleotide, fluorigenic aptamer, g-quadruplex, thioflavin t, corn mutant a14u, rna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12093.57

構造登録者

Sjekloca, L.,Ferre-D'Amare, A.R. (登録日: 2018-07-27, 公開日: 2019-07-31, 最終更新日: 2023-10-11)

主引用文献

Sjekloca, L.,Ferre-D'Amare, A.R.
Binding between G Quadruplexes at the Homodimer Interface of the Corn RNA Aptamer Strongly Activates Thioflavin T Fluorescence.
Cell Chem Biol, 26:1159-, 2019

PubMed Abstract: Thioflavin T (ThT) is widely used for the detection of amyloids. Many unrelated DNAs and RNAs that contain G-quadruplex motifs also bind ThT and strongly activate its fluorescence. To elucidate the structural basis of ThT binding to G quadruplexes and its fluorescence turn-on, we determined its co-crystal structure with the homodimeric RNA Corn, which contains two G quadruplexes. We found that two ThT molecules bind in the dimer interface, constrained by a G quartet from each protomer into a maximally fluorescent planar conformation. The unliganded Corn homodimer crystal structure reveals a collapsed fluorophore-binding site. In solution, Corn must fluctuate between this and an open, binding-competent conformation. A co-crystal structure with another benzothiazole derivate, thiazole orange (TO), also shows binding at the Corn homodimer interface. As the bound ThT and TO make no interactions with the RNA backbone, their Corn co-crystal structures likely explain their fluorescence activation upon sequence-independent DNA and RNA G-quadruplex binding.

PubMed: 31178406
DOI: 10.1016/j.chembiol.2019.04.012

実験手法

X-RAY DIFFRACTION (3.101 Å)