6DZC
Solution structure of Rbfox2 RRM mimetic peptide CPfox6
Summary for 6DZC
| Entry DOI | 10.2210/pdb6dzc/pdb |
| NMR Information | BMRB: 30489 |
| Descriptor | CPfox6 (1 entity in total) |
| Functional Keywords | peptidomimetics, peptide structure, rna recognition, rna binding protein |
| Biological source | synthetic construct |
| Total number of polymer chains | 1 |
| Total formula weight | 1935.26 |
| Authors | Sun, Y.-T.,Varani, G. (deposition date: 2018-07-03, release date: 2019-05-15, Last modification date: 2024-10-30) |
| Primary citation | Sun, Y.T.,Shortridge, M.D.,Varani, G. A Small Cyclic beta-Hairpin Peptide Mimics the Rbfox2 RNA Recognition Motif and Binds to the Precursor miRNA 20b. Chembiochem, 20:931-939, 2019 Cited by PubMed Abstract: The RNA recognition motif (RRM), which is the most abundant RNA-binding motif in eukaryotes, is a well-structured domain of about 90 amino acids, yet the β2β3 hairpin, corresponding to strands 2 and 3 of the β-sheet, and the intervening loop make essential interactions with RNA in many RRM complexes. A series of small cyclic peptide mimics of the β2β3 hairpin of Rbfox2 protein that recognize the terminal loop of precursor miR-20b have been designed to investigate whether the full RNA-binding protein can be mimicked with a minimal structurally preorganized peptide. Within a small library of seven cyclic peptides, a peptide with low-micromolar affinity for the miR-20b precursor was found. NMR spectroscopy titration data suggest that this peptide specifically targets the apical loop of pre-miR-20b. This work shows that it is possible to mimic RNA-binding proteins with designed stable peptides, which provide a starting point for designing or evolving small peptide mimetics of RRM proteins. PubMed: 30537200DOI: 10.1002/cbic.201800645 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
Download full validation report
