6DZB

Solution structure of Rbfox2 RRM mimetic peptide CPfox5

6DZB の概要

• エントリーDOI: 10.2210/pdb6dzb/pdb
• NMR情報: BMRB: 30488
• 分子名称: CPfox5 (1 entity in total)
• 機能のキーワード: peptidomimetics, peptide structure, rna recognition, rna binding protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1925.24

構造登録者

Sun, Y.-T.,Varani, G. (登録日: 2018-07-03, 公開日: 2019-05-15, 最終更新日: 2024-11-20)

主引用文献

Sun, Y.T.,Shortridge, M.D.,Varani, G.
A Small Cyclic beta-Hairpin Peptide Mimics the Rbfox2 RNA Recognition Motif and Binds to the Precursor miRNA 20b.
Chembiochem, 20:931-939, 2019

PubMed Abstract: The RNA recognition motif (RRM), which is the most abundant RNA-binding motif in eukaryotes, is a well-structured domain of about 90 amino acids, yet the β2β3 hairpin, corresponding to strands 2 and 3 of the β-sheet, and the intervening loop make essential interactions with RNA in many RRM complexes. A series of small cyclic peptide mimics of the β2β3 hairpin of Rbfox2 protein that recognize the terminal loop of precursor miR-20b have been designed to investigate whether the full RNA-binding protein can be mimicked with a minimal structurally preorganized peptide. Within a small library of seven cyclic peptides, a peptide with low-micromolar affinity for the miR-20b precursor was found. NMR spectroscopy titration data suggest that this peptide specifically targets the apical loop of pre-miR-20b. This work shows that it is possible to mimic RNA-binding proteins with designed stable peptides, which provide a starting point for designing or evolving small peptide mimetics of RRM proteins.

PubMed: 30537200
DOI: 10.1002/cbic.201800645

実験手法

SOLUTION NMR