6DSX

Bst DNA polymerase I post-chemistry (n+1 with dATP soak) structure

Summary for 6DSX

• Entry DOI: 10.2210/pdb6dsx/pdb
• Descriptor: DNA (5'-D(*GP*CP*GP*AP*TP*CP*AP*CP*GP*TP*A)-3'), DNA (5'-D(*GP*TP*AP*CP*GP*TP*GP*AP*TP*CP*GP*CP*A)-3'), DNA polymerase I, ... (6 entities in total)
• Functional Keywords: dna synthesis, transferase, transferase-dna complex, transferase/dna
• Biological source: Geobacillus stearothermophilus; More
• Total number of polymer chains: 3
• Total formula weight: 74376.57

Authors

Chim, N.,Jackson, L.N.,Chaput, J.C. (deposition date: 2018-06-14, release date: 2018-10-31, Last modification date: 2023-10-11)

Primary citation

Chim, N.,Jackson, L.N.,Trinh, A.M.,Chaput, J.C.
Crystal structures of DNA polymerase I capture novel intermediates in the DNA synthesis pathway.
Elife, 7:-, 2018

PubMed Abstract: High resolution crystal structures of DNA polymerase intermediates are needed to study the mechanism of DNA synthesis in cells. Here we report five crystal structures of DNA polymerase I that capture new conformations for the polymerase translocation and nucleotide pre-insertion steps in the DNA synthesis pathway. We suggest that these new structures, along with previously solved structures, highlight the dynamic nature of the finger subdomain in the enzyme active site.

PubMed: 30338759
DOI: 10.7554/eLife.40444

Experimental method

X-RAY DIFFRACTION (1.99 Å)