6DSU

Bst DNA polymerase I pre-insertion complex structure

6DSU の概要

• エントリーDOI: 10.2210/pdb6dsu/pdb
• 分子名称: DNA (5'-D(*GP*CP*GP*AP*TP*CP*AP*CP*GP*T)-3'), DNA (5'-D(P*AP*CP*GP*TP*GP*AP*TP*CP*GP*CP*A)-3'), DNA polymerase I, ... (7 entities in total)
• 機能のキーワード: dna synthesis, transferase, transferase-dna complex, transferase/dna
• 由来する生物種: Geobacillus stearothermophilus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 73439.84

構造登録者

Chim, N.,Jackson, L.N.,Chaput, J.C. (登録日: 2018-06-14, 公開日: 2018-10-31, 最終更新日: 2024-03-13)

主引用文献

Chim, N.,Jackson, L.N.,Trinh, A.M.,Chaput, J.C.
Crystal structures of DNA polymerase I capture novel intermediates in the DNA synthesis pathway.
Elife, 7:-, 2018

PubMed Abstract: High resolution crystal structures of DNA polymerase intermediates are needed to study the mechanism of DNA synthesis in cells. Here we report five crystal structures of DNA polymerase I that capture new conformations for the polymerase translocation and nucleotide pre-insertion steps in the DNA synthesis pathway. We suggest that these new structures, along with previously solved structures, highlight the dynamic nature of the finger subdomain in the enzyme active site.

PubMed: 30338759
DOI: 10.7554/eLife.40444

実験手法

X-RAY DIFFRACTION (1.98 Å)