6DQX

Actinobacillus ureae class Id ribonucleotide reductase alpha subunit

6DQX の概要

• エントリーDOI: 10.2210/pdb6dqx/pdb
• 分子名称: Ribonucleoside-diphosphate reductase, alpha chain, CHLORIDE ION, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: ribonucleotide reductase, alpha subunit, class id, nucleotide metabolism, oxidoreductase
• 由来する生物種: Actinobacillus ureae ATCC 25976
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 65703.38

構造登録者

McBride, M.J.,Palowitch, G.M.,Boal, A.K. (登録日: 2018-06-11, 公開日: 2019-04-17, 最終更新日: 2023-10-11)

主引用文献

Rose, H.R.,Maggiolo, A.O.,McBride, M.J.,Palowitch, G.M.,Pandelia, M.E.,Davis, K.M.,Yennawar, N.H.,Boal, A.K.
Structures of Class Id Ribonucleotide Reductase Catalytic Subunits Reveal a Minimal Architecture for Deoxynucleotide Biosynthesis.
Biochemistry, 58:1845-1860, 2019

PubMed Abstract: Class I ribonucleotide reductases (RNRs) share a common mechanism of nucleotide reduction in a catalytic α subunit. All RNRs initiate catalysis with a thiyl radical, generated in class I enzymes by a metallocofactor in a separate β subunit. Class Id RNRs use a simple mechanism of cofactor activation involving oxidation of a Mn cluster by free superoxide to yield a metal-based MnMn oxidant. This simple cofactor assembly pathway suggests that class Id RNRs may be representative of the evolutionary precursors to more complex class Ia-c enzymes. X-ray crystal structures of two class Id α proteins from Flavobacterium johnsoniae ( Fj) and Actinobacillus ureae ( Au) reveal that this subunit is distinctly small. The enzyme completely lacks common N-terminal ATP-cone allosteric motifs that regulate overall activity, a process that normally occurs by dATP-induced formation of inhibitory quaternary structures to prevent productive β subunit association. Class Id RNR activity is insensitive to dATP in the Fj and Au enzymes evaluated here, as expected. However, the class Id α protein from Fj adopts higher-order structures, detected crystallographically and in solution. The Au enzyme does not exhibit these quaternary forms. Our study reveals structural similarity between bacterial class Id and eukaryotic class Ia α subunits in conservation of an internal auxiliary domain. Our findings with the Fj enzyme illustrate that nucleotide-independent higher-order quaternary structures can form in simple RNRs with truncated or missing allosteric motifs.

PubMed: 30855138
DOI: 10.1021/acs.biochem.8b01252

実験手法

X-RAY DIFFRACTION (1.76 Å)