6DQL

Crystal structure of Regulator of Proteinase B RopB complexed with SIP

6DQL の概要

• エントリーDOI: 10.2210/pdb6dql/pdb
• 分子名称: Regulator of Proteinase B RopB, SpeB-inducing peptide (SIP) (3 entities in total)
• 機能のキーワード: streptococcus pyogenes, regulator, virulence regulation, quorum sensing, dna binding protein
• 由来する生物種: Streptococcus pyogenes; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 56742.26

構造登録者

Do, H.,Makthal, N.,VanderWal, A.R.,Olsen, R.J.,Musser, J.M.,Kumaraswami, M. (登録日: 2018-06-11, 公開日: 2019-05-15, 最終更新日: 2023-10-11)

主引用文献

Do, H.,Makthal, N.,VanderWal, A.R.,Saavedra, M.O.,Olsen, R.J.,Musser, J.M.,Kumaraswami, M.
Environmental pH and peptide signaling control virulence of Streptococcus pyogenes via a quorum-sensing pathway.
Nat Commun, 10:2586-2586, 2019

PubMed Abstract: Bacteria control gene expression in concert with their population density by a process called quorum sensing, which is modulated by bacterial chemical signals and environmental factors. In the human pathogen Streptococcus pyogenes, production of secreted virulence factor SpeB is controlled by a quorum-sensing pathway and environmental pH. The quorum-sensing pathway consists of a secreted leaderless peptide signal (SIP), and its cognate receptor RopB. Here, we report that the SIP quorum-sensing pathway has a pH-sensing mechanism operative through a pH-sensitive histidine switch located at the base of the SIP-binding pocket of RopB. Environmental acidification induces protonation of His144 and reorganization of hydrogen bonding networks in RopB, which facilitates SIP recognition. The convergence of two disparate signals in the SIP signaling pathway results in induction of SpeB production and increased bacterial virulence. Our findings provide a model for investigating analogous crosstalk in other microorganisms.

PubMed: 31197146
DOI: 10.1038/s41467-019-10556-8

実験手法

X-RAY DIFFRACTION (3.3 Å)