6DLB

Crystal Structure of an influenza A hemagglutinin antibody Fab CH65:1203d4 chimera

6DLB の概要

• エントリーDOI: 10.2210/pdb6dlb/pdb
• 分子名称: CH65:1203d4 Fab heavy chain, CH65:1203d4 Fab light chain, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: influenza a virus, hemagglutinin, antibody, antibody design, rosetta, immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47464.70

構造登録者

Dong, J.,Finn, J.A.,Crowe, J.E. (登録日: 2018-05-31, 公開日: 2019-06-05, 最終更新日: 2024-11-06)

主引用文献

Finn, J.A.,Dong, J.,Sevy, A.M.,Parrish, E.,Gilchuk, I.,Nargi, R.,Scarlett-Jones, M.,Reichard, W.,Bombardi, R.,Voss, T.G.,Meiler, J.,Crowe Jr., J.E.
Identification of Structurally Related Antibodies in Antibody Sequence Databases Using Rosetta-Derived Position-Specific Scoring.
Structure, 28:1124-1130.e5, 2020

PubMed Abstract: The amount of antibody (Ab) variable gene sequence information is expanding rapidly, but our ability to predict the function of Abs from sequence alone is limited. Here, we describe a sequence-to-function prediction method that couples structural data for a single Ab/antigen (Ag) complex with repertoire data. We used a position-specific structure-scoring matrix (P3SM) incorporating structure-prediction scores from Rosetta to identify Ab variable loops that have predicted structural similarity to the influenza virus-specific human Ab CH65. The P3SM approach identified new members of this Ab class. Recombinant Ab expression, crystallography, and virus inhibition assays showed that the HCDR3 loops of the newly identified Abs possessed similar structure and antiviral activity as the comparator CH65. This approach enables discovery of new human Abs with desired structure and function using cDNA repertoires that are obtained readily with current amplicon sequencing techniques.

PubMed: 32783953
DOI: 10.1016/j.str.2020.07.012

実験手法

X-RAY DIFFRACTION (2.2 Å)