6DKL

Crystal Structure of a Rationally Designed Six-Fold Symmetric DNA Scaffold

6DKL の概要

• エントリーDOI: 10.2210/pdb6dkl/pdb
• 分子名称: DNA (5'-D(P*GP*GP*AP*TP*GP*CP*AP*CP*A)-3'), DNA (5'-D(P*CP*AP*CP*AP*CP*CP*GP*TP*AP*C)-3'), DNA (5'-D(P*TP*GP*TP*GP*CP*TP*GP*TP*GP*CP*T)-3'), ... (4 entities in total)
• 機能のキーワード: dna nanotechnology, structural dna nanotechnology, self-assembly, dna, crystal design, dna crystals
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 12797.42

構造登録者

Simmons, C.R.,Zhang, F.,Yan, H. (登録日: 2018-05-29, 公開日: 2018-08-29, 最終更新日: 2024-03-13)

主引用文献

Zhang, F.,Simmons, C.R.,Gates, J.,Liu, Y.,Yan, H.
Self-Assembly of a 3D DNA Crystal Structure with Rationally Designed Six-Fold Symmetry.
Angew. Chem. Int. Ed. Engl., 57:12504-12507, 2018

PubMed Abstract: Programming self-assembled designer DNA crystals with various lattices and functions is one of the most important goals for nanofabrication using nucleic acids. The resulting porous materials possess atomic precision for several potential applications that rely on crystalline lattices and cavities. Herein, we present a rationally designed and self-assembled 3D DNA crystal lattice with hexagonal symmetry. In our design, two 21-base oligonucleotides are used to form a duplex motif that further assembles into a 3D array. The interactions between the strands are programmed using Watson-Crick base-pairing. The six-fold symmetry, as well as the chirality, is directed by the Holliday junctions formed between the duplex motifs. The rationally designed DNA crystal provides a new avenue that could create self-assembled macromolecular 3D crystalline lattices with atomic precision. In addition, the structure contains a highly organized array of well-defined cavities that are suitable for future applications with immobilized guests.

PubMed: 30066355
DOI: 10.1002/anie.201807223

実験手法

X-RAY DIFFRACTION (3.034 Å)