6DK7

RetS histidine kinase region with cobalt

Summary for 6DK7

• Entry DOI: 10.2210/pdb6dk7/pdb
• Related: 6DK8
• Descriptor: RetS (Regulator of Exopolysaccharide and Type III Secretion), COBALT (II) ION (3 entities in total)
• Functional Keywords: histidine kinase, inhibitor, signaling protein
• Biological source: Pseudomonas aeruginosa
• Total number of polymer chains: 8
• Total formula weight: 200817.33

Authors

Mancl, J.M.,Schubot, F.D. (deposition date: 2018-05-29, release date: 2019-03-20, Last modification date: 2024-03-13)

Primary citation

Mancl, J.M.,Ray, W.K.,Helm, R.F.,Schubot, F.D.
Helix Cracking Regulates the Critical Interaction between RetS and GacS in Pseudomonas aeruginosa.
Structure, 27:785-, 2019

PubMed Abstract: Recent paradigm shifting discoveries have demonstrated that bacterial signaling kinases engage in unexpected regulatory crosstalk, yet the underlying molecular mechanisms remain largely uncharacterized. The Pseudomonas aeruginosa RetS/GacS system constitutes an ideal model for studying these mechanisms. The in-depth analysis of the kinase region of RetS and RetS/GacS interactions presented here refutes a longstanding model, which posited the formation of a catalytically inactive RetS/GacS heterodimer. Crystallographic studies uncovered structurally dynamic features within the RetS kinase region, suggesting that RetS uses the reversible unfolding of a helix, or helix cracking, to control interactions with GacS. The pivotal importance of this helical region for regulating GacS and, by extension, Pseudomonas aeruginosa virulence, was corroborated via in vivo assays. The implications of this work extend beyond the RetS/GacS system because the helix cracking occurs right next to a highly conserved catalytic residue histidine-424, suggesting this model could represent an emergent archetype for histidine kinase regulation.

PubMed: 30879888
DOI: 10.1016/j.str.2019.02.006

Experimental method

X-RAY DIFFRACTION (2.6 Å)