6DK7

RetS histidine kinase region with cobalt

6DK7 の概要

• エントリーDOI: 10.2210/pdb6dk7/pdb
• 関連するPDBエントリー: 6DK8
• 分子名称: RetS (Regulator of Exopolysaccharide and Type III Secretion), COBALT (II) ION (3 entities in total)
• 機能のキーワード: histidine kinase, inhibitor, signaling protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 200817.33

構造登録者

Mancl, J.M.,Schubot, F.D. (登録日: 2018-05-29, 公開日: 2019-03-20, 最終更新日: 2024-03-13)

主引用文献

Mancl, J.M.,Ray, W.K.,Helm, R.F.,Schubot, F.D.
Helix Cracking Regulates the Critical Interaction between RetS and GacS in Pseudomonas aeruginosa.
Structure, 27:785-, 2019

PubMed Abstract: Recent paradigm shifting discoveries have demonstrated that bacterial signaling kinases engage in unexpected regulatory crosstalk, yet the underlying molecular mechanisms remain largely uncharacterized. The Pseudomonas aeruginosa RetS/GacS system constitutes an ideal model for studying these mechanisms. The in-depth analysis of the kinase region of RetS and RetS/GacS interactions presented here refutes a longstanding model, which posited the formation of a catalytically inactive RetS/GacS heterodimer. Crystallographic studies uncovered structurally dynamic features within the RetS kinase region, suggesting that RetS uses the reversible unfolding of a helix, or helix cracking, to control interactions with GacS. The pivotal importance of this helical region for regulating GacS and, by extension, Pseudomonas aeruginosa virulence, was corroborated via in vivo assays. The implications of this work extend beyond the RetS/GacS system because the helix cracking occurs right next to a highly conserved catalytic residue histidine-424, suggesting this model could represent an emergent archetype for histidine kinase regulation.

PubMed: 30879888
DOI: 10.1016/j.str.2019.02.006

実験手法

X-RAY DIFFRACTION (2.6 Å)