6DHR

NMR Solution structure of Rivi3

6DHR の概要

• エントリーDOI: 10.2210/pdb6dhr/pdb
• NMR情報: BMRB: 30470
• 分子名称: Rivi3 (1 entity in total)
• 機能のキーワード: cyclotide cyclic peptide, plant protein
• 由来する生物種: Rinorea virgata
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3139.78

構造登録者

Harvey, P.J.,Craik, D.J. (登録日: 2018-05-21, 公開日: 2019-04-17, 最終更新日: 2024-10-09)

主引用文献

Niyomploy, P.,Chan, L.Y.,Harvey, P.J.,Poth, A.G.,Colgrave, M.L.,Craik, D.J.
Discovery and Characterization of Cyclotides from Rinorea Species.
J. Nat. Prod., 81:2512-2520, 2018

PubMed Abstract: Cyclotides are macrocyclic cystine-knotted peptides most commonly found in the Violaceae plant family. Although Rinorea is the second-largest genera within the Violaceae family, few studies have examined whether or not they contain cyclotides. To further our understanding of cyclotide diversity and evolution, we examined the cyclotide content of two Rinorea species found in Southeast Asia: R. virgata and R. bengalensis. Seven cyclotides were isolated from R. virgata (named Rivi1-7), and a known cyclotide (cT10) was found in R. bengalensis. Loops 2, 5, and 6 of Rivi1-4 contained sequences not previously seen in corresponding loops of known cyclotides, thereby expanding our understanding of the diversity of cyclotides. In addition, the sequence of loop 2 of Rivi3 and Rivi4 were identical to some related noncyclic "acyclotides" from the Poaceae plant family. As only acyclotides, but not cyclotides, have been reported in monocotyledons thus far, our findings support an evolutionary link between monocotyledon-derived ancestral cyclotide precursors and dicotyledon-derived cyclotides. Furthermore, Rivi2 and Rivi3 had comparable cytotoxic activities to the most cytotoxic cyclotide known to date: cycloviolacin O2 from Viola odorata; yet, unlike cycloviolacin O2, they did not show hemolytic activity. Therefore, these cyclotides represent novel scaffolds for use in future anticancer drug design.

PubMed: 30387611
DOI: 10.1021/acs.jnatprod.8b00572

実験手法

SOLUTION NMR