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6DHB

Crystal structure of the human TIM-3 with bound Calcium

6DHB の概要
エントリーDOI10.2210/pdb6dhb/pdb
分子名称Hepatitis A virus cellular receptor 2, CALCIUM ION, BENZOIC ACID, ... (5 entities in total)
機能のキーワードt cell immunoglobulin mucin-3 immunological tolerance, immune system
由来する生物種Homo sapiens (Human)
タンパク質・核酸の鎖数1
化学式量合計12499.34
構造登録者
Gandhi, A.K.,Kim, W.M.,Huang, Y.H.,Bonsor, D.,Sundberg, E.,Sun, Z.-Y.,Petsko, G.A.,Kuchroo, V.,Blumberg, R.S. (登録日: 2018-05-19, 公開日: 2018-12-12, 最終更新日: 2024-10-30)
主引用文献Gandhi, A.K.,Kim, W.M.,Sun, Z.J.,Huang, Y.H.,Bonsor, D.A.,Sundberg, E.J.,Kondo, Y.,Wagner, G.,Kuchroo, V.K.,Petsko, G.,Blumberg, R.S.
High resolution X-ray and NMR structural study of human T-cell immunoglobulin and mucin domain containing protein-3.
Sci Rep, 8:17512-17512, 2018
Cited by
PubMed Abstract: T-cell immunoglobulin and mucin domain containing protein-3 (TIM-3) is an important immune regulator. Here, we describe a novel high resolution (1.7 Å) crystal structure of the human (h)TIM-3 N-terminal variable immunoglobulin (IgV) domain with bound calcium (Ca) that was confirmed by nuclear magnetic resonance (NMR) spectroscopy. Significant conformational differences were observed in the B-C, C'-C″ and C'-D loops of hTIM-3 compared to mouse (m)TIM-3, hTIM-1 and hTIM-4. Further, the conformation of the C-C' loop of hTIM-3 was notably different from hTIM-4. Consistent with the known metal ion-dependent binding of phosphatidylserine (PtdSer) to mTIM-3 and mTIM-4, the NMR spectral analysis and crystal structure of Ca-bound hTIM-3 revealed that residues in the hTIM-3 F-G loop coordinate binding to Ca. In addition, we established a novel biochemical assay to define hTIM-3 functionality as determined by binding to human carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1). These studies provide new insights useful for understanding and targeting hTIM-3.
PubMed: 30504845
DOI: 10.1038/s41598-018-35754-0
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (1.7 Å)
構造検証レポート
Validation report summary of 6dhb
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-01-15に公開中

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