6DGV

iGABASnFR Fluorescent GABA Sensor precursor

6DGV の概要

• エントリーDOI: 10.2210/pdb6dgv/pdb
• 分子名称: Fluorescent GABA Sensor precursor (2 entities in total)
• 機能のキーワード: gaba binding protein, fluorescent sensor, superfolder, circularly permuted gfp, periplasmic binding protein, fluorescent protein
• 由来する生物種: Pseudomonas fluorescens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 62633.34

構造登録者

Marvin, J.S.,Looger, L.L. (登録日: 2018-05-18, 公開日: 2019-04-03, 最終更新日: 2026-03-18)

主引用文献

Marvin, J.S.,Shimoda, Y.,Magloire, V.,Leite, M.,Kawashima, T.,Jensen, T.P.,Kolb, I.,Knott, E.L.,Novak, O.,Podgorski, K.,Leidenheimer, N.J.,Rusakov, D.A.,Ahrens, M.B.,Kullmann, D.M.,Looger, L.L.
A genetically encoded fluorescent sensor for in vivo imaging of GABA.
Nat.Methods, 16:763-770, 2019

PubMed Abstract: Current techniques for monitoring GABA (γ-aminobutyric acid), the primary inhibitory neurotransmitter in vertebrates, cannot follow transients in intact neural circuits. To develop a GABA sensor, we applied the design principles used to create the fluorescent glutamate receptor iGluSnFR. We used a protein derived from a previously unsequenced Pseudomonas fluorescens strain and performed structure-guided mutagenesis and library screening to obtain intensity-based GABA sensing fluorescence reporter (iGABASnFR) variants. iGABASnFR is genetically encoded, detects GABA release evoked by electric stimulation of afferent fibers in acute brain slices and produces readily detectable fluorescence increases in vivo in mice and zebrafish. We applied iGABASnFR to track mitochondrial GABA content and its modulation by an anticonvulsant, swimming-evoked, GABA-mediated transmission in zebrafish cerebellum, GABA release events during interictal spikes and seizures in awake mice, and found that GABA-mediated tone decreases during isoflurane anesthesia.

PubMed: 31308547
DOI: 10.1038/s41592-019-0471-2

実験手法

X-RAY DIFFRACTION (2.8 Å)