6DG2

Antigen Binding Fragment of the Pan-ebolavirus Monoclonal Antibody 6D6

Summary for 6DG2

• Entry DOI: 10.2210/pdb6dg2/pdb
• Descriptor: 6D6 Fab heavy chain, 6D6 Fab light chain (3 entities in total)
• Functional Keywords: antibody, monoclonal, antigen binding fragment, fab, ebola, immune system
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 4
• Total formula weight: 96755.61

Authors

Milligan, J.C.,Saphire, E.O. (deposition date: 2018-05-16, release date: 2018-09-26, Last modification date: 2024-10-23)

Primary citation

Milligan, J.C.,Parekh, D.V.,Fuller, K.M.,Igarashi, M.,Takada, A.,Saphire, E.O.
Structural Characterization of Pan-Ebolavirus Antibody 6D6 Targeting the Fusion Peptide of the Surface Glycoprotein.
J. Infect. Dis., 219:415-419, 2019

PubMed Abstract: Ebola virus infection causes severe disease in humans and represents a global health threat. Candidates for immunotherapeutics and vaccines have shown promise in clinical trials, although they are ineffective against other members of the Ebolavirus genus that also cause periodic, lethal outbreaks. In this study, we present a crystal structure of a pan-ebolavirus antibody, 6D6, as well as single-particle electron microscopy reconstructions of 6D6 in complex with Ebola and Bundibugyo virus glycoproteins. 6D6 binds to the conserved glycoprotein fusion peptide, implicating it as a site of immune vulnerability that could be exploited to reliably elicit a pan-ebolavirus neutralizing antibody response.

PubMed: 30203042
DOI: 10.1093/infdis/jiy532

Experimental method

X-RAY DIFFRACTION (1.96 Å)