6DD7

Crystal structure of plant UVB photoreceptor UVR8 from in situ serial Laue diffraction

6DD7 の概要

• エントリーDOI: 10.2210/pdb6dd7/pdb
• 分子名称: Ultraviolet-B receptor UVR8 (2 entities in total)
• 機能のキーワード: photoreceptor, laue diffraction, serial crystallography, in situ diffraction, gene regulation
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 163501.75

構造登録者

Ren, Z. (登録日: 2018-05-09, 公開日: 2018-07-18, 最終更新日: 2023-10-11)

主引用文献

Ren, Z.,Ayhan, M.,Bandara, S.,Bowatte, K.,Kumarapperuma, I.,Gunawardana, S.,Shin, H.,Wang, C.,Zeng, X.,Yang, X.
Crystal-on-crystal chips for in situ serial diffraction at room temperature.
Lab Chip, 18:2246-2256, 2018

PubMed Abstract: Recent developments in serial crystallography at X-ray free electron lasers (XFELs) and synchrotrons have been driven by two scientific goals in structural biology - first, static structure determination from nano or microcrystals of membrane proteins and large complexes that are difficult for conventional cryocrystallography, and second, direct observations of transient structural species in biochemical reactions at near atomic resolution. Since room-temperature diffraction experiments naturally demand a large quantity of purified protein, sample economy is critically important for all steps of serial crystallography from crystallization, crystal delivery to data collection. Here we report the development and applications of "crystal-on-crystal" devices to facilitate large-scale in situ serial diffraction experiments on protein crystals of all sizes - large, small, or microscopic. We show that the monocrystalline quartz as a substrate material prevents vapor loss during crystallization and significantly reduces background X-ray scattering. These devices can be readily adopted at XFEL and synchrotron beamlines, which enable efficient delivery of hundreds to millions of crystals to the X-ray beam, with an overall protein consumption per dataset comparable to that of cryocrystallography.

PubMed: 29952383
DOI: 10.1039/c8lc00489g

実験手法

X-RAY DIFFRACTION (2 Å)