6DCH

Structure of isonitrile biosynthesis enzyme ScoE

6DCH の概要

• エントリーDOI: 10.2210/pdb6dch/pdb
• 分子名称: ScoE protein, ACETATE ION, CHLORIDE ION, ... (6 entities in total)
• 機能のキーワード: isonitrile, non-heme iron, enzyme, metalloenzyme, oxidoreductase
• 由来する生物種: Streptomyces coeruleorubidus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37146.39

構造登録者

Born, D.A.,Drennan, C.L. (登録日: 2018-05-07, 公開日: 2018-06-27, 最終更新日: 2023-10-11)

主引用文献

Harris, N.C.,Born, D.A.,Cai, W.,Huang, Y.,Martin, J.,Khalaf, R.,Drennan, C.L.,Zhang, W.
Isonitrile Formation by a Non-Heme Iron(II)-Dependent Oxidase/Decarboxylase.
Angew. Chem. Int. Ed. Engl., 57:9707-9710, 2018

PubMed Abstract: The electron-rich isonitrile is an important functionality in bioactive natural products, but its biosynthesis has been restricted to the IsnA family of isonitrile synthases. We herein provide the first structural and biochemical evidence of an alternative mechanism for isonitrile formation. ScoE, a putative non-heme iron(II)-dependent enzyme from Streptomyces coeruleorubidus, was shown to catalyze the conversion of (R)-3-((carboxymethyl)amino)butanoic acid to (R)-3-isocyanobutanoic acid through an oxidative decarboxylation mechanism. This work further provides a revised scheme for the biosynthesis of a unique class of isonitrile lipopeptides, of which several members are critical for the virulence of pathogenic mycobacteria.

PubMed: 29906336
DOI: 10.1002/anie.201804307

実験手法

X-RAY DIFFRACTION (1.8 Å)