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6D7R

Crystal structure of an inactivate variant of the quorum-sensing master regulator HapR from protease-deficient non-O1, non-O139 Vibrio cholerae strain V2

Replaces:  5L0X
Summary for 6D7R
Entry DOI10.2210/pdb6d7r/pdb
DescriptorHemagglutinin/protease regulatory protein (2 entities in total)
Functional Keywordstetr, quorum-sensing, transcription
Biological sourceVibrio cholerae
Total number of polymer chains2
Total formula weight51903.60
Authors
Cruite, J.T.,Succo, P.,Raychaudhuri, S.,Kull, F.J. (deposition date: 2018-04-25, release date: 2018-05-30, Last modification date: 2024-11-20)
Primary citationCruite, J.,Succo, P.,Raychaudhuri, S.,Kull, F.J.
Crystal structure of an inactive variant of the quorum-sensing master regulator HapR from the protease-deficient non-O1, non-O139 Vibrio cholerae strain V2.
Acta Crystallogr F Struct Biol Commun, 74:331-336, 2018
Cited by
PubMed Abstract: HapR is a TetR-family transcriptional regulator that controls quorum sensing in Vibrio cholerae, the causative agent of cholera. HapR regulates the expression of hemagglutinin protease, virulence and biofilm genes. The crystal structure of wild-type HapR from V. cholerae strain O1 El Tor C6706 has previously been solved. In this study, the structure of a DNA-binding-deficient variant of HapR (HapR) derived from the protease-deficient V. cholerae serotype O37 strain V2 is reported. The structure reveals no structural differences compared with wild-type HapR. However, structural alignment of HapR with the TetR-family member QacR in complex with its operator DNA suggests that the aspartate residue located between the regulatory and DNA-binding domains may clash with and electrostatically repel the phosphate backbone of DNA to prevent binding.
PubMed: 29870016
DOI: 10.1107/S2053230X18006519
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.1 Å)
Structure validation

239803

数据于2025-08-06公开中

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