6D39

Photodissociable dimeric Dronpa green fluorescent protein variant V (pdDronpaV)

6D39 の概要

• エントリーDOI: 10.2210/pdb6d39/pdb
• 関連するPDBエントリー: 6D38
• 分子名称: Fluorescent protein Dronpa, SULFATE ION (3 entities in total)
• 機能のキーワード: dronpa, pddronpa, photosensory, gfp, dimer, photodissociable, fluorescent protein
• 由来する生物種: Echinophyllia sp. SC22
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29933.62

構造登録者

Zhou, X.,Fan, L.,Lin, M. (登録日: 2018-04-15, 公開日: 2018-05-23, 最終更新日: 2024-10-30)

主引用文献

Zhou, X.X.,Fan, L.Z.,Li, P.,Shen, K.,Lin, M.Z.
Optical control of cell signaling by single-chain photoswitchable kinases.
Science, 355:836-842, 2017

PubMed Abstract: Protein kinases transduce signals to regulate a wide array of cellular functions in eukaryotes. A generalizable method for optical control of kinases would enable fine spatiotemporal interrogation or manipulation of these various functions. We report the design and application of single-chain cofactor-free kinases with photoswitchable activity. We engineered a dimeric protein, pdDronpa, that dissociates in cyan light and reassociates in violet light. Attaching two pdDronpa domains at rationally selected locations in the kinase domain, we created the photoswitchable kinases psRaf1, psMEK1, psMEK2, and psCDK5. Using these photoswitchable kinases, we established an all-optical cell-based assay for screening inhibitors, uncovered a direct and rapid inhibitory feedback loop from ERK to MEK1, and mediated developmental changes and synaptic vesicle transport in vivo using light.

PubMed: 28232577
DOI: 10.1126/science.aah3605

実験手法

X-RAY DIFFRACTION (2.83 Å)