6CV0

Cryo-electron microscopy structure of infectious bronchitis coronavirus spike protein

6CV0 の概要

• エントリーDOI: 10.2210/pdb6cv0/pdb
• EMDBエントリー: 7631
• 関連するBIRD辞書のPRD_ID: PRD_900017
• 分子名称: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: infectious bronchitis coronavirus, spike, pre-fusion, cryo-em, viral protein
• 由来する生物種: Infectious bronchitis virus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 384631.80

構造登録者

Shang, J.,Zheng, Y.,Yang, Y.,Liu, C.,Geng, Q.,Luo, C.,Zhang, W.,Li, F. (登録日: 2018-03-27, 公開日: 2018-04-18, 最終更新日: 2024-11-06)

主引用文献

Shang, J.,Zheng, Y.,Yang, Y.,Liu, C.,Geng, Q.,Luo, C.,Zhang, W.,Li, F.
Cryo-EM structure of infectious bronchitis coronavirus spike protein reveals structural and functional evolution of coronavirus spike proteins.
PLoS Pathog., 14:e1007009-e1007009, 2018

PubMed Abstract: As cell-invading molecular machinery, coronavirus spike proteins pose an evolutionary conundrum due to their high divergence. In this study, we determined the cryo-EM structure of avian infectious bronchitis coronavirus (IBV) spike protein from the γ-genus. The trimeric IBV spike ectodomain contains three receptor-binding S1 heads and a trimeric membrane-fusion S2 stalk. While IBV S2 is structurally similar to those from the other genera, IBV S1 possesses structural features that are unique to different other genera, thereby bridging these diverse spikes into an evolutionary spectrum. Specifically, among different genera, the two domains of S1, the N-terminal domain (S1-NTD) and C-terminal domain (S1-CTD), diverge from simpler tertiary structures and quaternary packing to more complex ones, leading to different functions of the spikes in receptor usage and membrane fusion. Based on the above structural and functional comparisons, we propose that the evolutionary spectrum of coronavirus spikes follows the order of α-, δ-, γ-, and β-genus. This study has provided insight into the evolutionary relationships among coronavirus spikes and deepened our understanding of their structural and functional diversity.

PubMed: 29684066
DOI: 10.1371/journal.ppat.1007009

実験手法

ELECTRON MICROSCOPY (3.93 Å)