6CUM

Crystal structure of a C-terminal proteolytic fragment of a protein annotated as an LAO/AO transport system ATPase but likely MeaB and MMAA-like GTPase from Mycobacterium smegmatis

6CUM の概要

• エントリーDOI: 10.2210/pdb6cum/pdb
• 分子名称: LAO/AO transport system ATPase, 1,2-ETHANEDIOL (3 entities in total)
• 機能のキーワード: ssgcid, proteolytic fragment, structural genomics, seattle structural genomics center for infectious disease, transferase
• 由来する生物種: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32791.28

構造登録者

Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2018-03-26, 公開日: 2018-04-11, 最終更新日: 2024-04-03)

主引用文献

Abendroth, J.,Sankaran, B.,Myler, P.J.,Lorimer, D.D.,Edwards, T.E.
Ab initio structure solution of a proteolytic fragment using ARCIMBOLDO.
Acta Crystallogr F Struct Biol Commun, 74:530-535, 2018

PubMed Abstract: Crystal structure determination requires solving the phase problem. This can be accomplished using ab initio direct methods for small molecules and macromolecules at resolutions higher than 1.2 Å, whereas macromolecular structure determination at lower resolution requires either molecular replacement using a homologous structure or experimental phases using a derivative such as covalent labeling (for example selenomethionine or mercury derivatization) or heavy-atom soaking (for example iodide ions). Here, a case is presented in which crystals were obtained from a 30.8 kDa protein sample and yielded a 1.6 Å resolution data set with a unit cell that could accommodate approximately 8 kDa of protein. Thus, it was unclear what had been crystallized. Molecular replacement with pieces of homologous proteins and attempts at iodide ion soaking failed to yield a solution. The crystals could not be reproduced. Sequence-independent molecular replacement using the structures available in the Protein Data Bank also failed to yield a solution. Ultimately, ab initio structure solution proved successful using the program ARCIMBOLDO, which identified two α-helical elements and yielded interpretable maps. The structure was the C-terminal dimerization domain of the intended target from Mycobacterium smegmatis. This structure is presented as a user-friendly test case in which an unknown protein fragment could be determined using ARCIMBOLDO.

PubMed: 30198884
DOI: 10.1107/S2053230X18010063

実験手法

X-RAY DIFFRACTION (1.6 Å)