6CU1

X-ray structure of the S. typhimurium YrlA effector-binding module

6CU1 の概要

• エントリーDOI: 10.2210/pdb6cu1/pdb
• 分子名称: YrlA effector-binding module, MAGNESIUM ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: y rna, yrla, trna-like element, noncoding rna, rna
• 由来する生物種: Salmonella enterica subsp. enterica serovar Typhimurium
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26439.81

構造登録者

Wang, W.,Chen, X.,Wolin, S.L.,Xiong, Y. (登録日: 2018-03-23, 公開日: 2018-10-31, 最終更新日: 2024-03-13)

主引用文献

Wang, W.,Chen, X.,Wolin, S.L.,Xiong, Y.
Structural Basis for tRNA Mimicry by a Bacterial Y RNA.
Structure, 26:1635-, 2018

PubMed Abstract: Noncoding Y RNAs are present in both animal cells and many bacteria. In all species examined, Y RNAs tether the Ro60 protein to an effector protein to perform various cellular functions. Recently, a new Y RNA subfamily was identified in bacteria. Bioinformatic analyses of these YrlA (Y RNA-like A) RNAs predict that the effector-binding domain resembles tRNA. We present the structure of this domain, the overall folding of which is strikingly similar to canonical tRNAs. The tertiary interactions that are responsible for stabilizing tRNA are present in YrlA, making it a close tRNA mimic. However, YrlA lacks a free CCA end and contains a kink in the stem corresponding to the anticodon stem. Since nucleotides in the D and T stems are conserved among YrlAs, they may be an interaction site for an unknown factor. Our experiments identify YrlA RNAs as a new class of tRNA mimics.

PubMed: 30318468
DOI: 10.1016/j.str.2018.09.001

実験手法

X-RAY DIFFRACTION (3 Å)