6CRD

INFLUENZA VIRUS NEURAMINIDASE SUBTYPE N9 (TERN) with tetrabrachion (TB) domain stalk

6CRD の概要

• エントリーDOI: 10.2210/pdb6crd/pdb
• 関連するPDBエントリー: 7NN9
• 分子名称: Tetrabrachion,Neuraminidase, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: neuraminidase, sialidase, hydrolase(o-glucosyl), hydrolase-hydrolase, artificial stalk, tetrabrachion, hydrolase, viral protein-hydrolase complex, viral protein/hydrolase
• 由来する生物種: Staphylothermus marinus; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 448526.49

構造登録者

Streltsov, V.A.,Schmidt, P.,McKimm-Breschkin, J. (登録日: 2018-03-16, 公開日: 2019-01-23, 最終更新日: 2023-10-04)

主引用文献

Streltsov, V.A.,Schmidt, P.M.,McKimm-Breschkin, J.L.
Structure of an Influenza A virus N9 neuraminidase with a tetrabrachion-domain stalk.
Acta Crystallogr.,Sect.F, 75:89-97, 2019

PubMed Abstract: The influenza neuraminidase (NA) is a homotetramer with head, stalk, transmembrane and cytoplasmic regions. The structure of the NA head with a stalk has never been determined. The NA head from an N9 subtype influenza A virus, A/tern/Australia/G70C/1975 (H1N9), was expressed with an artificial stalk derived from the tetrabrachion (TB) tetramerization domain from Staphylothermus marinus. The NA was successfully crystallized both with and without the TB stalk, and the structures were determined to 2.6 and 2.3 Å resolution, respectively. Comparisons of the two NAs with the native N9 NA structure from egg-grown virus showed that the artificial TB stalk maintained the native NA head structure, supporting previous biological observations.

PubMed: 30713159
DOI: 10.1107/S2053230X18017892

実験手法

X-RAY DIFFRACTION (2.57 Å)