6CPB

Crystal structure of the heme domain of CooA from Carboxydothermus hydrogenoformans

6CPB の概要

• エントリーDOI: 10.2210/pdb6cpb/pdb
• 分子名称: Carbon monoxide oxidation system transcription regulator CooA-1, SULFATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: co sensing transcription regulator, heme binding protein, transcription, dna binding
• 由来する生物種: Carboxydothermus hydrogenoformans
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33502.26

構造登録者

Tripathi, S.M.,Poulos, T.L. (登録日: 2018-03-13, 公開日: 2018-05-16, 最終更新日: 2023-10-04)

主引用文献

Tripathi, S.,Poulos, T.L.
Testing the N-Terminal Velcro Model of CooA Carbon Monoxide Activation.
Biochemistry, 57:3059-3064, 2018

PubMed Abstract: CooAs are dimeric bacterial CO-sensing transcription factors that activate a series of enzymes responsible for CO oxidation. The crystal structure of Rhodospirillum rubrum (rrCooA) shows that the N-terminal Pro from monomer A of the dimer coordinates the heme of monomer B that locks rrCooA in the "off" state. When CO binds, it is postulated that the Pro is replaced with CO, resulting in a very large reorientation of the DNA binding domains required for specific binding to DNA. Crystal structures of the closely related CooA from Carboxydothermus hydrogenoformans (chCooA) are available, and in one of these, the CO-bound on-state indicates that the N-terminal region that is displaced when CO binds provides contacts between the heme and DNA binding domains that hold the DNA binding domain in position for DNA binding. This has been termed the N-terminal velcro model of CooA activation. The study presented here tests this hypothesis by generating a disulfide mutant that covalently locks chCooA in the on-state. A simple fluorescence assay was used to measure DNA binding, and the S-S mutant was found to be in the on-state even without CO. We also determined the high-resolution crystal structure of the apo-heme domain, and the resulting structure is very similar to the holo-heme-bound structure. This result shows that the heme binding motif forms a stable structure without heme or the DNA binding domain.

PubMed: 29708736
DOI: 10.1021/acs.biochem.8b00359

実験手法

X-RAY DIFFRACTION (1.155 Å)