6CP9

Contact-dependent growth inhibition toxin - immunity protein complex from Klebsiella pneumoniae 342

6CP9 の概要

• エントリーDOI: 10.2210/pdb6cp9/pdb
• 分子名称: CdiA, CdiI (3 entities in total)
• 機能のキーワード: toxin, rnase, structural genomics, psi-biology, midwest center for structural genomics, mcsg, structure-function analysis of polymorphic cdi toxin-immunity protein complexes, uc4cdi, toxin-antitoxin complex, protein structure initiative, toxin/antitoxin
• 由来する生物種: Klebsiella pneumoniae (strain 342); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 110058.12

構造登録者

Michalska, K.,Stols, L.,Eschenfeldt, W.,Hayes, C.S.,Goulding, C.W.,Joachimiak, A.,Midwest Center for Structural Genomics (MCSG),Structure-Function Analysis of Polymorphic CDI Toxin-Immunity Protein Complexes (UC4CDI) (登録日: 2018-03-13, 公開日: 2019-03-13, 最終更新日: 2024-11-13)

主引用文献

Gucinski, G.C.,Michalska, K.,Garza-Sanchez, F.,Eschenfeldt, W.H.,Stols, L.,Nguyen, J.Y.,Goulding, C.W.,Joachimiak, A.,Hayes, C.S.
Convergent Evolution of the Barnase/EndoU/Colicin/RelE (BECR) Fold in Antibacterial tRNase Toxins.
Structure, 27:1660-, 2019

PubMed Abstract: Contact-dependent growth inhibition (CDI) is a form of interbacterial competition mediated by CdiB-CdiA two-partner secretion systems. CdiA effector proteins carry polymorphic C-terminal toxin domains (CdiA-CT), which are neutralized by specific CdiI immunity proteins to prevent self-inhibition. Here, we present the crystal structures of CdiA-CT⋅CdiI complexes from Klebsiella pneumoniae 342 and Escherichia coli 3006. The toxins adopt related folds that resemble the ribonuclease domain of colicin D, and both are isoacceptor-specific tRNases that cleave the acceptor stem of deacylated tRNA. Although the toxins are similar in structure and substrate specificity, CdiA-CT activity requires translation factors EF-Tu and EF-Ts, whereas CdiA-CT is intrinsically active. Furthermore, the corresponding immunity proteins are unrelated in sequence and structure. CdiI forms a dimeric β sandwich, whereas CdiI is an α-solenoid monomer. Given that toxin-immunity genes co-evolve as linked pairs, these observations suggest that the similarities in toxin structure and activity reflect functional convergence.

PubMed: 31515004
DOI: 10.1016/j.str.2019.08.010

実験手法

X-RAY DIFFRACTION (2.55 Å)