6COR

CSP1-F11A

6COR の概要

• エントリーDOI: 10.2210/pdb6cor/pdb
• NMR情報: BMRB: 30430
• 分子名称: Competence-stimulating peptide type 1 (1 entity in total)
• 機能のキーワード: pneumococcus, quorum sensing, signaling molecule, csp, signaling protein
• 由来する生物種: Streptococcus pneumoniae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2172.64

構造登録者

Yang, Y. (登録日: 2018-03-12, 公開日: 2018-08-29, 最終更新日: 2024-05-15)

主引用文献

Yang, Y.,Cornilescu, G.,Tal-Gan, Y.
Structural Characterization of Competence-Stimulating Peptide Analogues Reveals Key Features for ComD1 and ComD2 Receptor Binding in Streptococcus pneumoniae.
Biochemistry, 57:5359-5369, 2018

PubMed Abstract: Streptococcus pneumoniae is an important pathogen that utilizes quorum sensing (QS) to regulate genetic transformation, virulence, and biofilm formation. The competence-stimulating peptide (CSP) is a 17-amino acid signal peptide that is used by S. pneumoniae to trigger QS. S. pneumoniae strains can be divided into two main specificity groups based on the CSP signal they produce (CSP1 or CSP2) and their compatible receptors (ComD1 or ComD2, respectively). Modulation of QS in S. pneumoniae can be achieved by targeting the CSP:ComD interaction using synthetic CSP analogues. However, to rationally design CSP-based QS modulators with enhanced activities, an in-depth understanding of the structural features that are required for receptor binding is needed. Herein, we report a comprehensive in-solution three-dimensional structural characterization of eight CSP1 and CSP2 analogues with varied biological activities using nuclear magnetic resonance spectroscopy. Analysis of these structures revealed two distinct hydrophobic patches required for effective ComD1 and ComD2 binding.

PubMed: 30125091
DOI: 10.1021/acs.biochem.8b00653

実験手法

SOLUTION NMR