6CMX

Human Teneurin 2 extra-cellular region

Summary for 6CMX

• Entry DOI: 10.2210/pdb6cmx/pdb
• EMDB information: 7526
• Related PRD ID: PRD_900118
• Descriptor: Teneurin-2, alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose, ... (5 entities in total)
• Functional Keywords: teneurin, membrane protein, cns
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 227465.99

Authors

Shalev-Benami, M.,Li, J.,Sudhof, T.,Skiniotis, G.,Arac, D. (deposition date: 2018-03-06, release date: 2018-07-25, Last modification date: 2025-06-04)

Primary citation

Li, J.,Shalev-Benami, M.,Sando, R.,Jiang, X.,Kibrom, A.,Wang, J.,Leon, K.,Katanski, C.,Nazarko, O.,Lu, Y.C.,Sudhof, T.C.,Skiniotis, G.,Arac, D.
Structural Basis for Teneurin Function in Circuit-Wiring: A Toxin Motif at the Synapse.
Cell, 173:735-748.e15, 2018

PubMed Abstract: Teneurins (TENs) are cell-surface adhesion proteins with critical roles in tissue development and axon guidance. Here, we report the 3.1-Å cryoelectron microscopy structure of the human TEN2 extracellular region (ECR), revealing a striking similarity to bacterial Tc-toxins. The ECR includes a large β barrel that partially encapsulates a C-terminal domain, which emerges to the solvent through an opening in the mid-barrel region. An immunoglobulin (Ig)-like domain seals the bottom of the barrel while a β propeller is attached in a perpendicular orientation. We further show that an alternatively spliced region within the β propeller acts as a switch to regulate trans-cellular adhesion of TEN2 to latrophilin (LPHN), a transmembrane receptor known to mediate critical functions in the central nervous system. One splice variant activates trans-cellular signaling in a LPHN-dependent manner, whereas the other induces inhibitory postsynaptic differentiation. These results highlight the unusual structural organization of TENs giving rise to their multifarious functions.

PubMed: 29677516
DOI: 10.1016/j.cell.2018.03.036

Experimental method

ELECTRON MICROSCOPY (3.1 Å)