6CLP

1.16 A MicroED structure of GSNQNNF at 2.5 e- / A^2

Summary for 6CLP

• Entry DOI: 10.2210/pdb6clp/pdb
• EMDB information: 7508
• Descriptor: GSNQNNF, ACETATE ION, ZINC ION, ... (4 entities in total)
• Functional Keywords: amyloid fibril, prion, zinc binding, protein fibril
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 904.21

Authors

Hattne, J.,Shi, D.,Glynn, C.,Zee, C.-T.,Gallagher-Jones, M.,Martynowycz, M.W.,Rodriguez, J.A.,Gonen, T. (deposition date: 2018-03-02, release date: 2018-05-16, Last modification date: 2024-03-13)

Primary citation

Hattne, J.,Shi, D.,Glynn, C.,Zee, C.T.,Gallagher-Jones, M.,Martynowycz, M.W.,Rodriguez, J.A.,Gonen, T.
Analysis of Global and Site-Specific Radiation Damage in Cryo-EM.
Structure, 26:759-766.e4, 2018

PubMed Abstract: Micro-crystal electron diffraction (MicroED) combines the efficiency of electron scattering with diffraction to allow structure determination from nano-sized crystalline samples in cryoelectron microscopy (cryo-EM). It has been used to solve structures of a diverse set of biomolecules and materials, in some cases to sub-atomic resolution. However, little is known about the damaging effects of the electron beam on samples during such measurements. We assess global and site-specific damage from electron radiation on nanocrystals of proteinase K and of a prion hepta-peptide and find that the dynamics of electron-induced damage follow well-established trends observed in X-ray crystallography. Metal ions are perturbed, disulfide bonds are broken, and acidic side chains are decarboxylated while the diffracted intensities decay exponentially with increasing exposure. A better understanding of radiation damage in MicroED improves our assessment and processing of all types of cryo-EM data.

PubMed: 29706530
DOI: 10.1016/j.str.2018.03.021

Experimental method

ELECTRON CRYSTALLOGRAPHY (1.16 Å)