6CL3

LyeTxI-b, a synthetic peptide derived from Lycosa erythrognatha spider venom, shows potent antibiotic activity, in vitro and in vivo

6CL3 の概要

• エントリーDOI: 10.2210/pdb6cl3/pdb
• NMR情報: BMRB: 30424
• 分子名称: Toxin LyeTx 1 (1 entity in total)
• 機能のキーワード: antimicrobial peptide, antimicrobial protein
• 由来する生物種: Lycosa erythrognatha (Spider)
• 細胞内の位置: Secreted : C0HJU9
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2725.41

構造登録者

de Lima, M.E.,dos Reis, P.V.,Resende, J.M.,Verly, R.M. (登録日: 2018-03-01, 公開日: 2018-05-30, 最終更新日: 2024-10-23)

主引用文献

Reis, P.V.M.,Boff, D.,Verly, R.M.,Melo-Braga, M.N.,Cortes, M.E.,Santos, D.M.,Pimenta, A.M.C.,Amaral, F.A.,Resende, J.M.,de Lima, M.E.
LyeTxI-b, a Synthetic Peptide Derived FromLycosa erythrognathaSpider Venom, Shows Potent Antibiotic Activityin Vitroandin Vivo.
Front Microbiol, 9:667-667, 2018

PubMed Abstract: The antimicrobial peptide LyeTxI isolated from the venom of the spider is a potential model to develop new antibiotics against bacteria and fungi. In this work, we studied a peptide derived from LyeTxI, named LyeTxI-b, and characterized its structural profile and its and antimicrobial activities. Compared to LyeTxI, LyeTxI-b has an acetylated N-terminal and a deletion of a His residue, as structural modifications. The secondary structure of LyeTxI-b is a well-defined helical segment, from the second amino acid to the amidated C-terminal, with no clear partition between hydrophobic and hydrophilic faces. Moreover, LyeTxI-b shows a potent antimicrobial activity against Gram-positive and Gram-negative planktonic bacteria, being 10-fold more active than the native peptide against LyeTxI-b was also active in an model of septic arthritis, reducing the number of bacteria load, the migration of immune cells, the level of IL-1β cytokine and CXCL1 chemokine, as well as preventing cartilage damage. Our results show that LyeTxI-b is a potential therapeutic model for the development of new antibiotics against Gram-positive and Gram-negative bacteria.

PubMed: 29681894
DOI: 10.3389/fmicb.2018.00667

実験手法

SOLUTION NMR