6CKS

Crystal Structure of BRD4 with QC4956

6CKS の概要

• エントリーDOI: 10.2210/pdb6cks/pdb
• 分子名称: Bromodomain-containing protein 4, 4-[5-(ethylsulfonyl)-2-methoxyphenyl]-2-methyl-6-(1-methyl-1H-pyrazol-4-yl)isoquinolin-1(2H)-one (3 entities in total)
• 機能のキーワード: brd4, acetyllysine, epigenetics, gene regulation-inhibitor complex, gene regulation/inhibitor
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Nucleus: O60885
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15449.81

構造登録者

Hosfield, D.J. (登録日: 2018-02-28, 公開日: 2018-05-02, 最終更新日: 2023-10-04)

主引用文献

Bennett, M.J.,Wu, Y.,Boloor, A.,Matuszkiewicz, J.,O'Connell, S.M.,Shi, L.,Stansfield, R.K.,Del Rosario, J.R.,Veal, J.M.,Hosfield, D.J.,Xu, J.,Kaldor, S.W.,Stafford, J.A.,Betancort, J.M.
Design, synthesis and biological evaluation of novel 4-phenylisoquinolinone BET bromodomain inhibitors.
Bioorg. Med. Chem. Lett., 28:1811-1816, 2018

PubMed Abstract: The bromodomain and extra-terminal (BET) family of epigenetic proteins has attracted considerable attention in drug discovery given its involvement in regulating gene transcription. Screening a focused small molecule library based on the bromodomain pharmacophore resulted in the identification of 2-methylisoquinoline-1-one as a novel BET bromodomain-binding motif. Structure guided SAR exploration resulted in >10,000-fold potency improvement for the BRD4-BD1 bromodomain. Lead compounds exhibited excellent potencies in both biochemical and cellular assays in MYC-dependent cell lines. Compound 36 demonstrated good physicochemical properties and promising exposure levels in exploratory PK studies.

PubMed: 29657099
DOI: 10.1016/j.bmcl.2018.04.016

実験手法

X-RAY DIFFRACTION (1.72 Å)