6CJD

NMR Structure of Salmonella Type III Secretion system protein OrgC

6CJD の概要

• エントリーDOI: 10.2210/pdb6cjd/pdb
• NMR情報: BMRB: 30417
• 分子名称: Putative cytoplasmic protein (1 entity in total)
• 機能のキーワード: controls needle assembly of t3ss, early substrate of t3ss, protein binding
• 由来する生物種: Salmonella enterica I
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14293.89

構造登録者

DEY, S.,DE GUZMAN, R.N. (登録日: 2018-02-26, 公開日: 2018-08-01, 最終更新日: 2024-05-15)

主引用文献

Kato, J.,Dey, S.,Soto, J.E.,Butan, C.,Wilkinson, M.C.,De Guzman, R.N.,Galan, J.E.
A protein secreted by theSalmonellatype III secretion system controls needle filament assembly.
Elife, 7:-, 2018

PubMed Abstract: Type III protein secretion systems (T3SS) are encoded by several pathogenic or symbiotic bacteria. The central component of this nanomachine is the needle complex. Here we show in a Typhimurium T3SS that assembly of the needle filament of this structure requires OrgC, a protein encoded within the T3SS gene cluster. Absence of OrgC results in significantly reduced number of needle substructures but does not affect needle length. We show that OrgC is secreted by the T3SS and that exogenous addition of OrgC can complement a mutation. We also show that OrgC interacts with the needle filament subunit PrgI and accelerates its polymerization into filaments in vitro. The structure of OrgC shows a novel fold with a shared topology with a domain from flagellar capping proteins. These findings identify a novel component of T3SS and provide new insight into the assembly of the type III secretion machine.

PubMed: 30015613
DOI: 10.7554/eLife.35886

実験手法

SOLUTION NMR