6CJ6

Structure of the poxvirus protein F9

6CJ6 の概要

• エントリーDOI: 10.2210/pdb6cj6/pdb
• 分子名称: Protein F9, S-1,2-PROPANEDIOL, 2-ETHOXYETHANOL, ... (16 entities in total)
• 機能のキーワード: entry fusion complex associated protein, viral protein
• 由来する生物種: Vaccinia virus (strain Western Reserve) (VACV)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 92217.32

構造登録者

Diesterbeck, U.S.,Gittis, A.G.,Garboczi, D.N.,Moss, B. (登録日: 2018-02-26, 公開日: 2019-03-06, 最終更新日: 2024-10-23)

主引用文献

Diesterbeck, U.S.,Gittis, A.G.,Garboczi, D.N.,Moss, B.
The 2.1 angstrom structure of protein F9 and its comparison to L1, two components of the conserved poxvirus entry-fusion complex.
Sci Rep, 8:16807-16807, 2018

PubMed Abstract: The poxvirus F9 protein is a component of the vaccinia virus entry fusion complex (EFC) which consists of 11 proteins. The EFC forms a unique apparatus among viral fusion proteins and complexes. We solved the atomic structure of the F9 ectodomain at 2.10 Å. A structural comparison to the ectodomain of the EFC protein L1 indicated a similar fold and organization, in which a bundle of five α-helices is packed against two pairs of β-strands. However, instead of the L1 myristoylation site and hydrophobic cavity, F9 possesses a protruding loop between α-helices α3 and α4 starting at Gly90. Gly90 is conserved in all poxviruses except Salmon gill poxvirus (SGPV) and Diachasmimorpha longicaudata entomopoxvirus. Phylogenetic sequence analysis of all Poxviridae F9 and L1 orthologs revealed the SGPV genome to contain the most distantly related F9 and L1 sequences compared to the vaccinia proteins studied here. The structural differences between F9 and L1 suggest functional adaptations during evolution from a common precursor that underlie the present requirement for each protein.

PubMed: 30429486
DOI: 10.1038/s41598-018-34244-7

実験手法

X-RAY DIFFRACTION (2.1 Å)