6CHJ

Wax ester synthase/diacylglycerol acyltransferase from Marinobacter aquaeolei VT8

6CHJ の概要

• エントリーDOI: 10.2210/pdb6chj/pdb
• 分子名称: Diacylglycerol O-acyltransferase, CHLORIDE ION (3 entities in total)
• 機能のキーワード: ws/dgat, wax ester synthase, diacylglycerol acyltransferase, transferase
• 由来する生物種: Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 103290.54

構造登録者

Petronikolou, N.,Satish, S.K. (登録日: 2018-02-22, 公開日: 2018-06-13, 最終更新日: 2024-04-03)

主引用文献

Petronikolou, N.,Nair, S.K.
Structural and Biochemical Studies of a Biocatalyst for the Enzymatic Production of Wax Esters.
Acs Catalysis, 8:6334-6344, 2018

PubMed Abstract: Wax esters are high-value products whose enzymatic synthesis is of increasing biotechnological interest. The fabrication of cell factories that mass-produce wax esters may provide a facile route towards a sustainable, and environment-friendly approach to a large-scale process for this commodity chemical. An expedient route for wax-ester biocatalysis may be facilitated by the action of enzymes termed wax ester synthases/diacylglycerol acyltransferases (WS/DGAT), which produce wax esters using fatty acids and alcohols as a precursor. In this work, we report the structure for a member of the WS/DGAT superfamily. The structural data in conjunction with bioinformatics and mutational analyses allowed us to identify the substrate binding pockets, and residues that may be important for catalysis. Using this information as a guide, we generated a mutant with preference towards shorter acyl-substrates. This study demonstrates the efficacy of a structure-guided engineering effort towards a WS/DGAT variant with preference towards wax esters of desired lengths.

PubMed: 31559109
DOI: 10.1021/acscatal.8b00787

実験手法

X-RAY DIFFRACTION (2.428 Å)