6CD7

Crystal structure of APH(2")-IVa in complex with plazomicin

「6C0C」から置き換えられました

6CD7 の概要

• エントリーDOI: 10.2210/pdb6cd7/pdb
• 分子名称: APH(2'')-Id, CHLORIDE ION, (2S)-4-amino-N-[(1R,2S,3S,4R,5S)-5-amino-4-{[(2S,3R)-3-amino-6-{[(2-hydroxyethyl)amino]methyl}-3,4-dihydro-2H-pyran-2-y l]oxy}-2-{[3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranosyl]oxy}-3-hydroxycyclohexyl]-2-hydroxybutanamide, ... (4 entities in total)
• 機能のキーワード: structural genomics, center for structural genomics of infectious diseases, csgid, eukaryotic protein kinase-like fold, aminoglycoside phosphotransferase, kinase, transferase, aminoglycosides, plazomicin, antibiotic, transferase-antibiotic complex, transferase/antibiotic
• 由来する生物種: Enterococcus casseliflavus (Enterococcus flavescens)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 71699.42

構造登録者

Stogios, P.J.,Evdokimova, E.,Dong, A.,Di Leo, R.,Savchenko, A.,Satchell, K.J.,Joachimiak, J.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2018-02-08, 公開日: 2018-02-28, 最終更新日: 2023-10-04)

主引用文献

Cox, G.,Ejim, L.,Stogios, P.J.,Koteva, K.,Bordeleau, E.,Evdokimova, E.,Sieron, A.O.,Savchenko, A.,Serio, A.W.,Krause, K.M.,Wright, G.D.
Plazomicin Retains Antibiotic Activity against Most Aminoglycoside Modifying Enzymes.
ACS Infect Dis, 4:980-987, 2018

PubMed Abstract: Plazomicin is a next-generation, semisynthetic aminoglycoside antibiotic currently under development for the treatment of infections due to multidrug-resistant Enterobacteriaceae. The compound was designed by chemical modification of the natural product sisomicin to provide protection from common aminoglycoside modifying enzymes that chemically alter these drugs via N-acetylation, O-adenylylation, or O-phosphorylation. In this study, plazomicin was profiled against a panel of isogenic strains of Escherichia coli individually expressing twenty-one aminoglycoside resistance enzymes. Plazomicin retained antibacterial activity against 15 of the 17 modifying enzyme-expressing strains tested. Expression of only two of the modifying enzymes, aac(2')-Ia and aph(2″)-IVa, decreased plazomicin potency. On the other hand, expression of 16S rRNA ribosomal methyltransferases results in a complete lack of plazomicin potency. In vitro enzymatic assessment confirmed that AAC(2')-Ia and APH(2'')-IVa (aminoglycoside acetyltransferase, AAC; aminoglycoside phosphotransferase, APH) were able to utilize plazomicin as a substrate. AAC(2')-Ia and APH(2'')-IVa are limited in their distribution to Providencia stuartii and Enterococci, respectively. These data demonstrate that plazomicin is not modified by a broad spectrum of common aminoglycoside modifying enzymes including those commonly found in Enterobacteriaceae. However, plazomicin is inactive in the presence of 16S rRNA ribosomal methyltransferases, which should be monitored in future surveillance programs.

PubMed: 29634241
DOI: 10.1021/acsinfecdis.8b00001

実験手法

X-RAY DIFFRACTION (1.53 Å)