6CCD

The crystal structure of Mycobacterium tuberculosis Rv1747 FHA-1

Summary for 6CCD

• Entry DOI: 10.2210/pdb6ccd/pdb
• Descriptor: ABC transporter ATP-binding/permease protein Rv1747, SULFATE ION (3 entities in total)
• Functional Keywords: fha domain, phospho-threonine binding domain, phospho-peptide binding domain, protein binding
• Biological source: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
• Total number of polymer chains: 1
• Total formula weight: 12424.92

Authors

Gay, L.M.,Gee, C.L.,Alber, T. (deposition date: 2018-02-06, release date: 2018-06-20, Last modification date: 2024-03-13)

Primary citation

Heinkel, F.,Shen, L.,Richard-Greenblatt, M.,Okon, M.,Bui, J.M.,Gee, C.L.,Gay, L.M.,Alber, T.,Av-Gay, Y.,Gsponer, J.,McIntosh, L.P.
Biophysical Characterization of the Tandem FHA Domain Regulatory Module from the Mycobacterium tuberculosis ABC Transporter Rv1747.
Structure, 26:972-, 2018

PubMed Abstract: The Mycobacterium tuberculosis ATP-binding cassette transporter Rv1747 is a putative exporter of cell wall biosynthesis intermediates. Rv1747 has a cytoplasmic regulatory module consisting of two pThr-interacting Forkhead-associated (FHA) domains connected by a conformationally disordered linker with two phospho-acceptor threonines (pThr). The structures of FHA-1 and FHA-2 were determined by X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy, respectively. Relative to the canonical 11-strand β-sandwich FHA domain fold of FHA-1, FHA-2 is circularly permuted and lacking one β-strand. Nevertheless, the two share a conserved pThr-binding cleft. FHA-2 is less stable and more dynamic than FHA-1, yet binds model pThr peptides with moderately higher affinity (∼50 μM versus 500 μM equilibrium dissociation constants). Based on NMR relaxation and chemical shift perturbation measurements, when joined within a polypeptide chain, either FHA domain can bind either linker pThr to form intra- and intermolecular complexes. We hypothesize that this enables tunable phosphorylation-dependent multimerization to regulate Rv1747 transporter activity.

PubMed: 29861345
DOI: 10.1016/j.str.2018.04.018

Experimental method

X-RAY DIFFRACTION (1.8 Å)