6CBF

Macrophage Migration Inhibitory Factor in Complex with a Pyrazole Inhibitor (6a)

6CBF の概要

• エントリーDOI: 10.2210/pdb6cbf/pdb
• 分子名称: Macrophage migration inhibitory factor, 2-phenoxy-5-(1H-pyrazol-4-yl)benzoic acid, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: inhibitor, complex, cytokine
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 37913.91

構造登録者

Robertson, M.J.,Krimmer, S.G.,Jorgensen, W.L. (登録日: 2018-02-02, 公開日: 2018-04-04, 最終更新日: 2024-03-13)

主引用文献

Trivedi-Parmar, V.,Robertson, M.J.,Cisneros, J.A.,Krimmer, S.G.,Jorgensen, W.L.
Optimization of Pyrazoles as Phenol Surrogates to Yield Potent Inhibitors of Macrophage Migration Inhibitory Factor.
ChemMedChem, 13:1092-1097, 2018

PubMed Abstract: Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that is implicated in the regulation of inflammation, cell proliferation, and neurological disorders. MIF is also an enzyme that functions as a keto-enol tautomerase. Most potent MIF tautomerase inhibitors incorporate a phenol, which hydrogen bonds to Asn97 in the active site. Starting from a 113-μm docking hit, we report results of structure-based and computer-aided design that have provided substituted pyrazoles as phenol alternatives with potencies of 60-70 nm. Crystal structures of complexes of MIF with the pyrazoles highlight the contributions of hydrogen bonding with Lys32 and Asn97, and aryl-aryl interactions with Tyr36, Tyr95, and Phe113 to the binding.

PubMed: 29575754
DOI: 10.1002/cmdc.201800158

実験手法

X-RAY DIFFRACTION (2.3 Å)