6CAY
Crystal structure of the first StART-like domain of Ysp2p/Lam2p in its apo and ergosterol-bound state.
Summary for 6CAY
| Entry DOI | 10.2210/pdb6cay/pdb |
| Descriptor | Sterol-binding protein, ERGOSTEROL (3 entities in total) |
| Functional Keywords | membrane contact sites, lipid transport protein, cholesterol, start domain, endoplasmic reticulum, lipid binding protein |
| Biological source | Saccharomyces cerevisiae (Baker's yeast) |
| Total number of polymer chains | 4 |
| Total formula weight | 77435.48 |
| Authors | Horenkamp, F.A.,Valverde, D.P.,Reinisch, K.M. (deposition date: 2018-02-01, release date: 2018-02-28, Last modification date: 2024-11-13) |
| Primary citation | Horenkamp, F.A.,Valverde, D.P.,Nunnari, J.,Reinisch, K.M. Molecular basis for sterol transport by StART-like lipid transfer domains. EMBO J., 37:-, 2018 Cited by PubMed Abstract: Lipid transport proteins at membrane contact sites, where two organelles are closely apposed, play key roles in trafficking lipids between cellular compartments while distinct membrane compositions for each organelle are maintained. Understanding the mechanisms underlying non-vesicular lipid trafficking requires characterization of the lipid transporters residing at contact sites. Here, we show that the mammalian proteins in the lipid transfer proteins anchored at a membrane contact site (LAM) family, called GRAMD1a-c, transfer sterols with similar efficiency as the yeast orthologues, which have known roles in sterol transport. Moreover, we have determined the structure of a lipid transfer domain of the yeast LAM protein Ysp2p, both in its apo-bound and sterol-bound forms, at 2.0 Å resolution. It folds into a truncated version of the steroidogenic acute regulatory protein-related lipid transfer (StART) domain, resembling a lidded cup in overall shape. Ergosterol binds within the cup, with its 3-hydroxy group interacting with protein indirectly via a water network at the cup bottom. This ligand binding mode likely is conserved for the other LAM proteins and for StART domains transferring sterols. PubMed: 29467216DOI: 10.15252/embj.201798002 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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