6C5Y

Crystal structure of thaumatin from microcrystals

Summary for 6C5Y

• Entry DOI: 10.2210/pdb6c5y/pdb
• Descriptor: Thaumatin-1 (2 entities in total)
• Functional Keywords: microcrystal, microdiffraction, data analysis, plant protein
• Biological source: Thaumatococcus daniellii (Katemfe)
• Total number of polymer chains: 1
• Total formula weight: 22227.06

Authors

Guo, G.,Fuchs, M.,Shi, W.,Skinner, J.,Berman, E.,Ogata, C.M.,Hendrickson, W.A.,McSweeney, S.,Liu, Q. (deposition date: 2018-01-17, release date: 2018-05-30, Last modification date: 2023-10-04)

Primary citation

Guo, G.,Fuchs, M.R.,Shi, W.,Skinner, J.,Berman, E.,Ogata, C.M.,Hendrickson, W.A.,McSweeney, S.,Liu, Q.
Sample manipulation and data assembly for robust microcrystal synchrotron crystallography.
IUCrJ, 5:238-246, 2018

PubMed Abstract: With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo-graphy with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. The method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines.

PubMed: 29755741
DOI: 10.1107/S2052252518005389

Experimental method

X-RAY DIFFRACTION (2.5 Å)