6C5Y

Crystal structure of thaumatin from microcrystals

6C5Y の概要

• エントリーDOI: 10.2210/pdb6c5y/pdb
• 分子名称: Thaumatin-1 (2 entities in total)
• 機能のキーワード: microcrystal, microdiffraction, data analysis, plant protein
• 由来する生物種: Thaumatococcus daniellii (Katemfe)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22227.06

構造登録者

Guo, G.,Fuchs, M.,Shi, W.,Skinner, J.,Berman, E.,Ogata, C.M.,Hendrickson, W.A.,McSweeney, S.,Liu, Q. (登録日: 2018-01-17, 公開日: 2018-05-30, 最終更新日: 2024-11-20)

主引用文献

Guo, G.,Fuchs, M.R.,Shi, W.,Skinner, J.,Berman, E.,Ogata, C.M.,Hendrickson, W.A.,McSweeney, S.,Liu, Q.
Sample manipulation and data assembly for robust microcrystal synchrotron crystallography.
IUCrJ, 5:238-246, 2018

PubMed Abstract: With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo-graphy with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. The method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines.

PubMed: 29755741
DOI: 10.1107/S2052252518005389

実験手法

X-RAY DIFFRACTION (2.5 Å)