6C41

The clavanin peptide in the presence of TFE (2,2,2-trifluoroethanol), presented a amphipathic alpha-helices from Phe-2 to Val-22 residues

6C41 の概要

• エントリーDOI: 10.2210/pdb6c41/pdb
• NMR情報: BMRB: 30396
• 分子名称: Clavanin-A (1 entity in total)
• 機能のキーワード: antimicrobial peptides, biofilm, antimicrobial protein
• 由来する生物種: Styela clava (Sea squirt)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2670.12

構造登録者

Alves, E.S.F.,Liao, L.M. (登録日: 2018-01-11, 公開日: 2018-02-07, 最終更新日: 2024-11-13)

主引用文献

Silva, O.N.,Alves, E.S.,de la Fuente-Nunez, C.,Ribeiro, S.M.,Mandal, S.M.,Gaspar, D.,Veiga, A.S.,Castanho, M.A.,Andrade, C.A.,Nascimento, J.M.,Fensterseifer, I.C.,Porto, W.F.,Correa, J.R.,Hancock, R.E.,Korpole, S.,Oliveira, A.L.,Liao, L.M.,Franco, O.L.
Structural Studies of a Lipid-Binding Peptide from Tunicate Hemocytes with Anti-Biofilm Activity.
Sci Rep, 6:27128-, 2016

PubMed Abstract: Clavanins is a class of peptides (23aa) histidine-rich, free of post-translational modifications. Clavanins have been studied largely for their ability to disrupt bacterial membranes. In the present study, the interaction of clavanin A with membranes was assessed by dynamic light scattering, zeta potential and permeabilization assays. We observed through those assays that clavanin A lysis bacterial cells at concentrations corresponding to its MIC. Further, the structure and function of clavanin A was investigated. To better understand how clavanin interacted with bacteria, its NMR structure was elucidated. The solution state NMR structure of clavanin A in the presence of TFE-d3 indicated an α-helical conformation. Secondary structures, based on circular dichroism measurements in anionic sodium dodecyl sulfate (SDS) and TFE (2,2,2-trifluorethanol), in silico lipid-peptide docking and molecular simulations with lipids DPPC and DOPC revealed that clavanin A can adopt a variety of folds, possibly influencing its different functions. Microcalorimetry assays revealed that clavanin A was capable of discriminating between different lipids. Finally, clavanin A was found to eradicate bacterial biofilms representing a previously unrecognized function.

PubMed: 27292548
DOI: 10.1038/srep27128

実験手法

SOLUTION NMR