6C28

Transcriptional repressor, CouR, bound to p-coumaroyl-CoA

6C28 の概要

• エントリーDOI: 10.2210/pdb6c28/pdb
• 分子名称: Transcriptional regulator, MarR family, p-coumaroyl-CoA (3 entities in total)
• 機能のキーワード: transcriptional regulation, repressor, marr family, dna binding protein
• 由来する生物種: Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 84668.60

構造登録者

Cogan, D.P.,Nair, S.K. (登録日: 2018-01-07, 公開日: 2018-05-30, 最終更新日: 2024-03-13)

主引用文献

Cogan, D.P.,Baraquet, C.,Harwood, C.S.,Nair, S.K.
Structural basis of transcriptional regulation by CouR, a repressor of coumarate catabolism, inRhodopseudomonas palustris.
J. Biol. Chem., 293:11727-11735, 2018

PubMed Abstract: The MarR family transcriptional regulator CouR, from the soil bacterium CGA009, has recently been shown to negatively regulate a -coumarate catabolic operon. Unlike most characterized MarR repressors that respond to small metabolites at concentrations in the millimolar range, repression by CouR is alleviated by the 800-Da ligand -coumaroyl-CoA with high affinity and specificity. Here we report the crystal structures of ligand-free CouR as well as the complex with -coumaroyl-CoA, each to 2.1-Å resolution, and the 2.85-Å resolution cocrystal structure of CouR bound to an oligonucleotide bearing the cognate DNA operator sequence. In combination with binding experiments that uncover specific residues important for ligand and DNA recognition, these structures provide glimpses of a MarR family repressor in all possible states, providing an understanding of the molecular basis of DNA binding and the conformation alterations that accompany ligand-induced dissociation for activation of the operon.

PubMed: 29794028
DOI: 10.1074/jbc.RA118.003561

実験手法

X-RAY DIFFRACTION (2.09 Å)