6C22

Capsid protein in the Staphylococcus aureus phage 80alpha-derived SaPI1 mature capsid

6C22 の概要

• エントリーDOI: 10.2210/pdb6c22/pdb
• EMDBエントリー: 7332 7333
• 分子名称: Major head protein (1 entity in total)
• 機能のキーワード: major capsid protein, hk97-like fold, mature capsid, virus
• 由来する生物種: Staphylococcus virus 80alpha
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 147387.53

構造登録者

Kizziah, J.L.,Dearborn, A.D.,Dokland, T. (登録日: 2018-01-05, 公開日: 2018-01-17, 最終更新日: 2024-03-13)

主引用文献

Kizziah, J.L.,Manning, K.A.,Dearborn, A.D.,Wall, E.A.,Klenow, L.,Hill, R.L.L.,Spilman, M.S.,Stagg, S.M.,Christie, G.E.,Dokland, T.
Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80 alpha and SaPI1 Capsids.
Viruses, 9:-, 2017

PubMed Abstract: In the tailed bacteriophages, DNA is packaged into spherical procapsids, leading to expansion into angular, thin-walled mature capsids. In many cases, this maturation is accompanied by cleavage of the major capsid protein (CP) and other capsid-associated proteins, including the scaffolding protein (SP) that serves as a chaperone for the assembly process. bacteriophage 80α is capable of high frequency mobilization of mobile genetic elements called pathogenicity islands (SaPIs), such as SaPI1. SaPI1 redirects the assembly pathway of 80α to form capsids that are smaller than those normally made by the phage alone. Both CP and SP of 80α are N-terminally processed by a host-encoded protease, Prp. We have analyzed phage mutants that express pre-cleaved or uncleavable versions of CP or SP, and show that the N-terminal sequence in SP is absolutely required for assembly, but does not need to be cleaved in order to produce viable capsids. Mutants with pre-cleaved or uncleavable CP display normal viability. We have used cryo-EM to solve the structures of mature capsids from an 80α mutant expressing uncleavable CP, and from wildtype SaPI1. Comparisons with structures of 80α and SaPI1 procapsids show that capsid maturation involves major conformational changes in CP, consistent with a release of the CP N-arm by SP. The hexamers reorganize during maturation to accommodate the different environments in the 80α and SaPI1 capsids.

PubMed: 29258203
DOI: 10.3390/v9120384

実験手法

ELECTRON MICROSCOPY (8.4 Å)